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. 2018 Mar 7;6(1):17–32. doi: 10.1016/j.jcmgh.2018.02.013

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© 2018 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Effects of gastrin siRNA polyplex on growth of pancreatic cancer in vitro. (A) Effects of siRNA-loaded NPs on growth of pancreatic cancer in vitro. Growth of pancreatic cancer cells PANC-1 (A1) and AsPC-1 (A2) was decreased significantly with the addition of polyplex loaded with the gastrin siRNA compared with scrambled control RNA. (B) Localization of polyplex in cancer cells after uptake. Confocal microscopy (Zeiss) images of BxPC-3 human pancreatic cancer cells in culture treated with PBS (control, B1) or polyplex loaded with fluorescently labeled Cy3 siRNA shows uptake and localization of the siRNA in the cancer cell cytoplasm when treated with 240 nmol/L (B2) or 480 nmol/L (B3) of siRNA. (C) Measurement of target mRNA after treatment with siRNA-loaded polyplex. Quantitative RT-PCR showed a dose-dependent decrease in gastrin mRNA in AsPC-1 (C1) and BxPC-3 (C2) cells treated with polyplex carrying gastrin siRNA at 120, 240, or 480 nmol/L, but not in vehicle controls. (D) Analysis of gastrin peptide expression by immunofluorescence in AsPC-1 (D1) and PANC-1 (D2) cells after treatment with siRNA polyplex confirms that the polyplex loaded with gastrin siRNA also decreased peptide expression.