Fig. 4.

Confocal immunofluorescent imaging of ADSCs in the presence of MDA MB 231 TCM. ADSCs treated with MDA MB 231 TCM ± 250 pg/mL sFRP4 at Day 10. Expression of a α-SMA, b vimentin. Left panel of each image shows a broad field of view captured using 10X objective and the right panel shows a normal field of view captured using 10X objective of a Nikon confocal microscope. c and d show quantitative measurement of fluorescent intensity of α-SMA and vimentin respectively, measured using the captured immunofluorescent images and analysed using Image J software