. 2018 Jan 6;11(1):85–92. doi: 10.1007/s12307-017-0203-z

Table 2.

Apoptosis-related miRNA target selection and expression analysis

					Cell line
					TOV-21G		SKOV-3
miRNA selected	Predicted targets related to apoptosis	Prediction Database	Validation Database	Taqman Assay ID	Log₂(FC)**	Interpretation	Log₂(FC)	Interpretation
hsa-miR-150-5p	TP53, AIFM2	TargetScan, miRanda	miRTarBase	000473	−0.003	*Sub expressed (p < 0.01)	−4.094	*Sub expressed (p < 0.01)
hsa-miR-181a	DDX3X, BCL2	TargetScan, miRanda	TARBASE (DianaTools)	002317	−2.932	p = 0.498	−3.580	*Sub expressed (p < 0.01)
hsa-miR-340-5p	DDX5, BCL2L2	TargetScan	TARBASE (DianaTools)	002258	−1.797	*Sub expressed (p < 0.01)	−4.645	*Sub expressed (p < 0.01)
hsa-miR-128	ACIN1, AFF1	TargetScan,	miRTarBase	002216	−3.777	*Sub expressed (p < 0.01)	−5.090	*Sub expressed (p < 0.01)
hsa-miR-125b-5p	BAK1, BCL2	TargetScan	TARBASE (DianaTools)	000449	−1.999	*Sub expressed (p < 0.01)	−7.722	*Sub expressed (p < 0.01)
hsa-miR-26a	BID, THAP5	TargetScan	TARBASE (DianaTools)	002443	0.558	p = 0.502	−2.866	*Sub expressed (p < 0.01)
hsa-miR-181c	BCL2, BCL2L11	TargetScan, miRanda	miRTarBase	000482	1.426	p = 0.502	0.445	*Superexpressed (p < 0.01)
hsa-miR-20a	BCL2, BCL2L11, THAP1	TargetScan	TARBASE (DianaTools)	000580	−2.056	*Sub expressed (p < 0.01)	−4.792	*Sub expressed (p < 0.01)
hsa-miR-221	BMF, APAF1	TargetScan	miRTarBase	000524	−0.746	p = 0.498	−9.155	*Sub expressed (p < 0.01)
hsa-miR-17-5p	CAAP1, CASP8, AREL1	MirTarget2	–	002308	−0.795	*Sub expressed (p < 0.01)	−14.744	*Sub expressed (p < 0.01)
hsa-miR-4478	APAF1, TNFRSF10C, TNFRSF10B	MirTarget2	–	465302_mat	−1.636	*Sub expressed (p < 0.01)	−5.974	*Sub expressed (p < 0.01)

Software: REST 2009 (Qiagen). *Significantly different (α = 5%), comparing tumorspheres to monolayer model, n = 2

**Fold Change (FC) = (2^–ΔΔCq)