Table 2.

Primer sequences of the genes amplified for expression analysis

Name of marker	Primer sequences (Forward and Reverse)
E-cadherin	forward: 5’ACGTCGTAATCACCACACTGA3’ reverse:5’TTCGTCACTGCTACGTGTAGAA3’
N-cadherin	forward:5’ACAGTGGCCACCTACAAAGG3’ reverse:5’CCGAGATGGGGTTGATAATG3’
Vimentin	forward:5’CTTCGCCAACTACATCGACA3’ reverse:5’GCTTCAACGGCAAAGTTCTC3’
Slug	forward:5’TCGGACCCACACATTACC3’ reverse:5’CCGAGATGGGGTTGATAATG3’
Snail	forward:5’TCGTCCTTCTCCTCTACTTC3’ reverse:5’TTCCTTGTTGCAGTATTTGC3’
Zeb	forward:5’CTGAAGAGGACCAGAGGCAG3’ reverse:5’CCCAGAACTGCGTCACATGTC3’
Twist	forward: 5’AGCTGAGCAAGATTCAGACCCTC3’ reverse: 5’CCGTCTGGGAATCACTGT3’
MMP-2	forward:5’GGATGATGCCTTTGCTCG3’ reverse:5’ATAGGATGTGCCCTGGAA3’
MMP-7	forward:5’GTGGTCACCTACAGGATCGT3’ reverse:5’ACCATCCGTCCAGCGTTCAT3’
MMP-9	forward: 5’AGGACGGCAATGCTGATC3’ reverse: 5’TCGTAGTTGGCGGTGGTG 3’
β-actin	forward:5’GGACTTCGAGCAAGAGATGG3’ reverse:5’AGCACTGTGTTGGCGTACAG3’