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. 2018 Jun 9;18:25–32. doi: 10.1016/j.redox.2018.06.003

Fig. 1.

Fig. 1

Treatment of cardiac cells with adropin reduces PDK4 expression and PDH phosphorylation. Treatment of H9c2 cardiac cells with 0.5 μg/mL adropin for 4 h does not have a significant effect on basal respiration or glycolysis in Seahorse XF assays (A-B). N = 12. Exposure of cells to adropin for 0–8 h leads to a reduction in Pdk4 expression, while levels of Ppargc1a, Cd36 and Cpt1b remain unchanged (C-F). The reduction in PDK4 protein abundance results in a decrease in PDH phosphorylation at serine 293 after 24 h (G-H). N = 3–4. * = P < 0.05.