Fig. 2.

Genetic depletion of the putative adropin receptor GPR19 promotes PDK4 expression and blocks adropin-mediated PDK4 downregulation. Expression of the putative adropin receptor, Gpr19, is not regulated by exposure to changing nutrient levels (low glucose = 5.5 mM, high glucose = 25 mM) or 0.5 μg/mL adropin over 0–24 h (A-B) in H9c2 cells. Knockdown of Gpr19 using siRNA led to a concomitant increase in Pdk4 gene and protein expression (C-E). Knockdown of Gpr19 prevented the reduction of Pdk4 expression seen in control cells treated with adropin for 4 h (F). N = 3–4, * = P < 0.05 all vs. Con siRNA+Veh; # = P < 0.05 Con siRNA+Adr vs. GPR19 siRNA+Veh.