Figure 1.

(A) Experimental timeline. Mice were intranasally administered a cigarette smoke solution (CSS) between days -23 and day 0 (five cycles, each including four daily doses of 20 μL/mouse and one rest day). Subsequently, the olfactory epithelium (OE) was collected for immunohistochemistry (IHC) and quantitative real-time polymerase chain reaction (qPCR) on the days indicated. (B,C) Representative images of hematoxylin and eosin stained sections of the OE from untreated mice (B, 40× magnification; (C) 400× magnification). The box in (B) indicates the region of the OE shown at higher magnification in (C).