Figure 3.

CXCR3⁺ Treg cells express a specific set of co-inhibitory receptors and cytotoxic molecules. Differential expression of a selection of genes coding for co-inhibitory receptors and granzymes was determined in pooled spleens and LNs from naïve or day 14 LCMV-infected mice. (A,C) Transcriptional expression levels were quantified by qPCR in sorted CXCR3⁺, CXCR3⁻, naïve Treg cells, and CD4⁺Foxp3-GFP⁻ effector T cells and (B,D) protein levels in CXCR3⁺ and CXCR3⁻ Treg cells were determined by flow cytometry (n.d., not detected).