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. 2018 Jun 13;9:1321. doi: 10.3389/fimmu.2018.01321

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Copyright © 2018 Miyazawa, Murata, Matsuura, Shibasaki, Yabu and Nakanishi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Dual fluorescence analysis of CD3ε⁺, Zap-70 with lymphocyte markers in tissues. Leukocytes from spleen, kidney, and thymus were stained with the anti-CD4-1 and CD8α mAbs followed by Alexa Fluor^® 488 anti-rat IgG, and stained with anti-gCD3ε Ab or anti-hZap-70 mAb followed by 647 goat anti-rabbit IgG. (A–C) Lymphocytes were gated on FS and SS dot plot. (D) Leukocytes from peripheral blood leukocytes were stained with anti-CD4-1, CD8α, IgM, phagocyte, and thrombocyte mAbs, respectively, followed by Alexa Fluor^® 488 goat anti-rat or mouse IgG, and stained with anti-gCD3ε Ab followed by Alexa Fluor^® 647 goat anti-rabbit IgG. Mean ± SD of more than three independent experiments are shown.

Dual fluorescence analysis of CD3ε⁺, Zap-70 with lymphocyte markers in tissues. Leukocytes from spleen, kidney, and thymus were stained with the anti-CD4-1 and CD8α mAbs followed by Alexa Fluor^® 488 anti-rat IgG, and stained with anti-gCD3ε Ab or anti-hZap-70 mAb followed by 647 goat anti-rabbit IgG. (A–C) Lymphocytes were gated on FS and SS dot plot. (D) Leukocytes from peripheral blood leukocytes were stained with anti-CD4-1, CD8α, IgM, phagocyte, and thrombocyte mAbs, respectively, followed by Alexa Fluor^® 488 goat anti-rat or mouse IgG, and stained with anti-gCD3ε Ab followed by Alexa Fluor^® 647 goat anti-rabbit IgG. Mean ± SD of more than three independent experiments are shown.