Figure 7.
Effects of in vitro culture on CD3ε and Zap-70 expression in kidney lymphocytes. (A) Kidney leukocytes were cultured for 24 h and the expression of CD3ε was analyzed by FACS with more than 1 × 105 of lymphocytes. Mean value and ±SD of more than three independent experiments are shown. (B) Average percentages of CD3ε and Zap-70 were calculated (n = 3, mean + SD). Statistical significance was calculated using t tests (***p < 0.005) (ns, not significant p > 0.05). (C) Total RNA was prepared from 2 × 106 cultured cells and used for quantitative RT-PCR analysis. mRNA expression of cd3e in cultured cells was normalized to T cell control gene, lck. Average percentages of CD3ε and Zap-70 were calculated (n = 3, mean + SD). Statistical significance was calculated using one-way ANOVA (ns, not significant p > 0.05).