Table 1.
Oligonucleotide primers used in this study.
| Primer name | Sequence(5′ → 3′) | GenBank accession number | |
|---|---|---|---|
| cDNA cloning | |||
| gcd3eF1 | GAAGCCGGAAAAGATGTCAG | 3′RACE | |
| gcd3eF2 | CAGTGAAACCGAAGAACAGC | 3′RACE | |
| gcd3eR1 | GGTTTGGGGGACGAGGAG | 5′RACE | |
| gcd3eR2 | TCTCATAGTCCGGGTTTGG | 5′RACE | |
| gcd3eR3 | GAGTCTGAGTGTTCAGTTTCTCATA | 5′RACE | |
| gcd3e-CDS F | TGTCTTCAGGACGATACAGAACC | CDS | |
| gcd3e-CDS R | CCCTATGAATCACCAGAGTTTGA | CDS | |
| gcd3e-del-TM-F | TGTTCTGCCAGAAACAGTGACAGCAAACC | TM deletion | |
| gcd3e-del-TM-R | TCCGCTCAACTCATAGCAGTTCTCACAC | TM deletion | |
| Eukaryotic expression | |||
| gcd3e-rec F | CATATGATCTGCACTGGAGGAGACAATAGGGTAG | Recombinant protein | |
| gcd3e-rec R | CATATGCTACTTATTGAGGCCTGCGTACAACCCATC | Recombinant protein | |
| Expression analysis | |||
| gef1αF | ACCCCAAGGCTCTCAATCT | qPCR | AB491676 |
| gef1αR | TCAACGCTCTTGATGACACC | qPCR | |
| gcd3eF | CTGCTATGAGTTGAGCGGAGTGAT | qPCR | LC378416 |
| gcd3eR | CTTCGGTTTGCTGTCACTGTTTCT | qPCR | |
| gtcrbF | CCTGAAGCCCTCTGAAATCG | qPCR | AB186399 |
| gtcrbR | TGCTTCCAAGGCTCCATCTT | qPCR | |
| gzap70F | TCCGAGAGAGAGAAGAATTTGGA | qPCR | |
| gzap70R | CATTGCGTATTTCCCTGATTTGT | qPCR | |
| glckF | CCATCCAGTCAAATACAGCAAA | qPCR | AB279594 |
| glckR | CTTTCTCAAACCCAAGGTCATC | qPCR | |
| In situ hybridization (ISH) | |||
| gcd3e probe-t7 | TAATACGACTCACTATAGGGGATGTCAGTGGAGAAGGAAA | ISH | |
| gcd3e probe-sp6 | ATTTAGGTGACACTATAGAAGTCATGTATATCTCTCAGTG | ISH | |