Figure 1.

miR-494 downregulation occurs in neuroblastoma (NB) differentiation and modify cell response to H₂O₂. (A) Expression levels of mature miR-494 in undifferentiated or all-trans retinoic acid (ATRA)-differentiated SH-SY5Y and SK-N-BE(2C) NB cells. hsa-miR-425-5p and hsa-let7g-5p were used as endogenous reference miRs. Results are reported as relative to the values obtained in untreated control cells, which was set equal to 1. Statistical analysis: n = 3; *p < 0.05 vs undifferentiated. (B) Expression levels of mature miR-128 in undifferentiated or ATRA-differentiated SH-SY5Y and SK-N-BE(2C) NB cells. hsa-miR-425-5p and hsa-let7g-5p were used as endogenous reference miRs. Results are reported as relative to the values obtained in untreated control cells, which was set equal to 1. Statistical analysis: n = 3. No significant differences. (C) WB analysis of PTEN. GAPDH expression has been used as loading control. 40 µg of protein has been loaded. The bands show the most representative experiment. Statistical analysis: n = 3; *p < 0.05 vs NegC. (D) WB analysis of hnRNPQ. GAPDH expression has been used as loading control. 40 µg of protein has been loaded. The bands show the most representative experiment. Statistical analysis: n = 2; *p < 0.05 vs NegC. (E) Percentage of viable cells (Trypan blue analysis) after miR-494 inhibition and 24 h exposure to 500 µM H₂O₂. Statistical analysis: n = 4; *p < 0.05 vs NegC and miRNA 494 inhibitor.