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. 2018 Jun 13;9:1215. doi: 10.3389/fmicb.2018.01215

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Copyright © 2018 Vicario, Mattiolo, Montini, Piano, Cavallari, Amadori, Chieco-Bianchi and Calabrò.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Morphologic and viral characterization of C91/PL and C91/III cell lines. (A) Phase contrast images of C91/PL and C91/III cells in standard culture conditions. The tumorigenic C91/III cell line showed a higher percentage of multinucleated giant cells. Original magnification 100×; Upper right-end side insert 200×. (B) Profile of Human T cell Leukemia Virus type 1 (HTLV-1) transcripts. C91/III cells showed a statistically significant increase in the normalized copy number (NCN) of all transcripts encoding the viral accessory proteins compared to C91/PL cells. NCN values were calculated by normalizing the absolute copy number of each transcript for the copy number of the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) transcripts. Values are the mean of three independent measurements performed in triplicate; standard error bars are shown. ^∗p < 0.05, ^∗∗p < 0.01.