Fig. 3.

L2-Cmu preferentially improves female healthspan. Following 6 mo of mAb treatment, several functional measures of healthspan were assessed, including exercise tolerance in female [Young (n = 8), Old Con (n = 16), and Old mAb (n = 15)] and male mice [Young (n = 10), Old Con (n = 9), and Old mAb (n = 9)], grip strength in female [Young (n = 8), Old Con (n = 12), and Old mAb (n = 9)] and male mice [Young (n = 10), Old Con (n = 10), and Old mAb (n = 9)], and balance beam in female [Young (n = 8), Old Con (n = 12), and Old mAb (n = 15)] and male mice [Young (n = 8), Old Con (n = 9), and Old mAb (n = 9)]. a–c In females, mAb led to significant improvements in exercise tolerance (~50% over Old Con), grip strength (two-fold over Old Con), and motor coordination. d–f In males, L2-Cmu improved exercise tolerance by ~28%, but did not lead to improvements in grip strength, while motor coordination was only modestly improved on a medium difficulty beam. Bars represent mean ± s.e.m. Dot plots overlaid on bar graphs represent individual data points. Different letters denote a significant difference between groups by Tukey HSD, P ≤ 0.05