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. 2018 Jun 19;8:9350. doi: 10.1038/s41598-018-27548-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TasA protein is essential for dextran-induced B. subtilis aggregation. B. subtilis cells were centrifuged and washed twice using aggregation buffer. The bacterial cells were incubated in aggregation buffer supplemented with different concentrations of dextran. O.D (600 nm) measurements were taken every 30 min. Final aggregation percentages were calculated. The data are displayed as a mean value ± standard deviation of data from three biological repeats each performed as duplicates. ANOVA following post-hoc t-test with Bonferroni correction has been conducted. *P value < 0.05 compared to control.