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. 2018 Jun 19;8:9350. doi: 10.1038/s41598-018-27548-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of the tapA and eps operon in B. subtilis are up regulated in the presence of S. mutans. B. subtilis (P_tapA-lacZ or P_eps-lacZ) cells were grown in the presence or absence of S. mutans cells either in pH7 or in pH6. Samples were collected every 12 hours for β galactosidaze assay. The data are displayed as a mean value ± standard deviation of data from three biological repeats each performed as triplicates. The expression of the tasA and the eps operons was significantly induced in the presence of S. mutans. The expression time of both of the operons was not in parallel. While tasA expression was significantly increased after the first 12 h of incubation, the higher induction in eps expression was after 36 h. *P value < 0.05 compared to B. subtilis pH = 7.