Fig. 1.

Expression of RIPK4 in BC cell lines and tissues. a The levels of the RIPK4 protein in five BC cell lines examined by western blot. b, c RIPK4 expression increased in BC tissues, compared to the paired adjacent normal bladder urothelial tissues specimens from 15 patients, determined by qRT-PCR analysis and western blot analysis. d Representative immunohistochemistry images show the low expression of RIPK4 in one BC tissue sample and high expression of RIPK4 in three BC tissue samples. Original magnification, ×200. e–l Kaplan–Meier curves with univariate analyses for patients with low RIPK4 expression versus high RIPK4 expression BC tumours. e Kaplan–Meier survival curves comparing cumulative overall survival rates in all patients with BC with low and high RIPK4 expression levels. f Survival curves for patients with low-grade disease. g Survival curves for patients with high-grade disease. h Survival curves for patients with pTa/pT1 disease. i Survival curves for patients with pT2 disease. j Survival curves for patients with pT3/pT4 disease. k Survival curves for patients with pN- disease. l Survival curves for patients with pN + disease