Table 2.
APP-mPCR1 primers for detection of serovars 1–12 and 15.
| Primer name | Sequence | Target genea | Amplicon size | Reference or source |
|---|---|---|---|---|
| AP1F | CTGGAGTAATTACGGCGACTATTCC | cps1B | 959 | Bossé et al. (2014) |
| AP1R | AGGAGAAGCTAGTAGTACTTGCATTTTC | cps1B | ||
| AP2F | GAGTGTGATGATGATGCTCTGGTTC | cps2E | 247 | Bossé et al. (2014) |
| AP2R | TACCAATAACTGTTGCAACTAACGC | cps2E | ||
| AP3F | TTGTAGAGCCCGCCAGATTTACG | cps3F | 500 | This study |
| AP3R | CATTCGCACCAGCAATCACC | cps3F | ||
| AP4F | CAGCATGGGTTTGGTCCTGTTG | cps4B | 204 | This study |
| AP4R | GGCTTTCTCCGTGTATGAATAAAGTG | cps4B | ||
| AP5F | AGCCACAAGACCCGAATGGTATAATG | cps5B | 825 | Bossé et al. (2014) |
| AP5R | CCATCAAATGCAGCTTCAAGGAGC | cps5B | ||
| AP6F | TGACTGGCTTCGTGAAAATGAG | cps6F | 718 | This study |
| AP6R | GTCTGAAGTTTTATTCGCAGCTCC | cps6F | ||
| AP7F | TTGGAATGGATTCATGATTGGGC | cps7E | 601 | Bossé et al. (2014) |
| AP7R | CGGAAATGGCCTATTGAAAAACG | cps7E | ||
| AP8F | ACATCCAAGCCGTTCTCCAG | cps8F | 1126 | This study |
| AP8R | CATCCATGAGCCAATGAGGG | cps8G | ||
| AP9F | GTAGGACGTGGTAACATTGAGGC | cps9E | 2105 | This study |
| AP9R | ACGGGTGCAATTTCTAAAGCTG | cps9F | ||
| AP10F | GGTGGTGATGGAACAAGGTTATGG | cps10A | 183 | Bossé et al. (2014) |
| AP10R | CTGTAATTGATGCGAAATAGTAGATTGGTGC | cps10A | ||
| AP12F | TAAAGGTATTATAACGCCGGCTCT | cps12A | 347 | Bossé et al. (2014) |
| AP12R | CTCCCATCTGTTGTCTAAGTAGTAG | cps12A | ||
| AP15F | GCAACTTGGAGAACATGGTTAAATCAAG | cps15B | 1595 | This study |
| AP15R | CAACCCTCCAATGTAAGCGAAGG | cps15C | ||
| apxIVA1 | TTATCCGAACTTTGGTTTAGCC | apxIV | 418 | Zhou et al. (2008) |
| apxIVA3 | CATATTTGATAAAACCATCCGTC | Intergenicb |
a
For each serovar capsule locus, the biosynthetic genes are labeled alphabetically starting from A in each case.
b
Intergenic region immediately downstream of apxIVA.