Table 3.
APP-mPCR2 for detection of serovars 13, 14, and 16–18.
| Primer name | Sequence | Target genea | Amplicon size | Reference or source |
|---|---|---|---|---|
| AP13F | GTTGTGTATCGAGGTTGGCATTTC | cps13E | 665 | This study |
| AP13R | ATGTAAAGGATCTAAGCCGTGTG | cps13E | ||
| AP14F | TGCATTACGCTTATATTCTGAATGG | cps14G | 1911 | This study |
| AP14R | TTGTCGATCGAGAGGGAGTAACG | ydeN | ||
| AP16F | TTACTCACTTGGGCTAGGGATAG | cps16C | 212 | Bossé et al. (2017) |
| AP16R | ACCAGCAATATGATTACGCCC | cps16D | ||
| AP17F | TTGTAATGGCGGTGTAATGCTAC | cps17F | 302 | Bosse et al. (2018) |
| AP17R | CATAAGTGCAGCCATCTCTTTCAG | cps17F | ||
| AP18F | CGGAGTTTGGCAGCATAAAGG | cps18B | 514 | Bosse et al. (2018) |
| AP18R | CCATAATCGGTGCTCAACTAAGAATG | cps18B | ||
| nadVF | CTCACTAAACAAAACTCTGCGTTC | nadV | 1339 | This study |
| nadVR | TTCGGATGACAGAACTTTTACCCG | nadV | ||
| apxIVA1 | TTATCCGAACTTTGGTTTAGCC | apxIV | 418 | Zhou et al. (2008) |
| apxIVA3 | CATATTTGATAAAACCATCCGTC | Intergenicb |
a
For each serovar capsule locus, the biosynthetic genes are labelled alphabetically starting from A in each case.
b
Intergenic region immediately downstream of apxIVA.