Fig. 1.

Effects of TBBPA and TCDD on adipocyte differentiation in hMSCs.

hMSCs were incubated with 1, 3.3, and 10 μM TBBPA and/or 0.0, 0.1, 0.3, and 1 nM TCDD for 21 days. (A) Lipid droplets were stained with oil red O. Morphological changes were observed under a microscope at 40× magnification. (B) Quantitative measurement of lipid droplets stained with oil red O. Oil red O in lipid droplets was eluted with isopropanol. The relative absorbance at 550 nm was expressed as the fold induction as compared with the vehicle. After incubation, aP2 (C), LPL (D), PPARγ (E), and C/EBPα (F) mRNA levels were measured by quantitative real-time PCR. The relative mRNA level was expressed as the fold induction as compared with the vehicle. The data are presented as means ± SD (n = 5). *P < 0.05, **P < 0.01, compared with vehicle-treated cells in 0.0 nM TCDD. #P < 0.05, ##P < 0.01, compared with vehicle-treated cells in 0.1 nM TCDD. †P < 0.05, ††P < 0.01, compared with vehicle-treated cells in 0.3 nM TCDD. §P < 0.05, §§P < 0.01, compared with vehicle-treated cells in 1.0 nM TCDD.