FIGURE 6.

The LytR contributes to TA biosynthesis in S. pneumoniae. (A) Teichoic acids were analyzed using Western blot and Dot blot, in which TEPC-15 was used to probe the TAs. GADPH was used to indicate the equivalent of the sample loading. Lane 1: wild-type D39; lane 2: lytR mutant; lane 3: complemented strain; lane 4: lytR mutant grown in C+Y medium plus LytR protein. Three independent experiments were performed and representative figures were presented. (B) TAs in bacterial lysates and supernatant were determined using ELISA. Three independent experiments were performed and the data represent means of three replicates ± standard deviations. For bacterial lysates, R6 WT vs R6ΔlytR, P = 0.0061; R6ΔlytR vs R6ΔlytR-C, P = 0.02. ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.0001.