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. 2018 Jun 11;9:1317. doi: 10.3389/fimmu.2018.01317

Table 4.

Amino acid distributions in CDRs 1–3.

CDR1 (IMGT 27–38) CDR2 (IMGT 56–65) CDR3 (IMGT 105–117), 12–15AA



SynVH-C (%) SynVH-SD (%) SynVH-C (%) SynVH-SD (%) SynVH-C (%) SynVH-SD (%)
D 5.24 12.16 5.04 4.44 11.50 11.64
E 0.05 0.00 0.59 0.28 2.08 1.02
K 0.12 0.15 0.26 1.16 6.53 5.56
R 1.53 0.57 2.43 1.54 4.51 3.80
H 2.68 0.71 0.60 0.32 0.60 3.46
T 12.11 11.98 12.80 14.07 5.94 4.01
S 17.70 14.85 23.92 23.46 8.05 3.15
N 3.80 3.10 5.02 9.72 6.89 4.84
Q 0.00 0.04 0.06 0.05 0.17 2.29
G 14.74 13.67 23.94 19.35 6.56 4.25*
A 6.39 2.10 6.06 1.56 8.84 9.64
C 0.00 0.03 0.04 0.00 0.05 1.28
P 0.43 0.47 0.51 0.15 1.24 1.80
V 0.56 0.65 2.13 1.03 2.14 10.32*
I 1.20 0.32 14.52 14.85 1.74 1.73
L 0.78 0.37 0.69 0.28 1.26 1.96
M 0.04 0.00 0.06 0.08 0.32 8.25*
F 24.26 24.11 0.17 0.09 14.65* 1.03
Y 8.35 11.43 1.14 3.39 10.90 18.59
W 0 3.27 0.04 4.17 6.05 1.38

IMGT CDR designations were used. For CDR-H3, amino acid frequencies in CDR lengths of 12–15 residues were calculated and averaged. Asterisk (*) indicates those CDR3 residues that are specific to either JH4 (SynVH-C) or JH6 (SynVH-SD). Only the human sequences were analyzed from the six SynVH-C birds (n = 5,652) and three SynVH-SD birds (n = 3,000).