Figure 10.

L-mDC co-cultured with heterologous total CD4⁺ T-cells reveal IL-10-dependent proliferation suppression, increased IFN-γ production, and Treg expansion. (A) L-mDC proliferation suppression in total CD4⁺ T-cells mixed co-cultures is IL-10-dependent. Antigen-presenting cells, mDCs, or L-mDCs, were co-cultured with heterologous total CD4⁺ T-cells (ratio 1:10) for 5 days in the presence of an isotype control (left) or an anti-IL-10 (5 µg/ml) monoclonal antibody (right). (B) Total CD4⁺ T-cells produce higher levels of IFN-γ when stimulated with L-mDC. After 5 days of co cultures, cells were stimulated for 4 h with PMA/Ionomycin in the presence of brefeldin; then, the cells were harvested and intracellularly stained with an anti-IFN-γ monoclonal antibody. (C) L-mDCs expand higher numbers of regulatory CD4⁺CD25^high FoxP3^high T-cells after 5 days of co-culture with total CD4⁺ cells (**p < 0.03).