Figure 2.

CD1A and CD1D genes are counter-regulated in L-iDCs. The increase in CD1D expression results in enhanced recognition of L-iDCs by NKT cells. (A) Relative quantification of CD1A and CD1D gene transcript levels in control dendritic cells (DCs) and L-DCs. Data (mean ± SDs) from four independent experiments are shown. (B) α-GalCer-loaded L-iDCs or iDCs were co-cultured with previously purified NKT cells at two different ratios for 4 h. A lactate dehydrogenase detection kit was used to measure the lysis of DCs. The average of four independent experiments is shown (*p < 0.05). (C) Expression of VICAM-I and ICAM-I on DCs. A representative from four experiments is shown.