Skip to main content
. 2018 Jun 13;9:775. doi: 10.3389/fpls.2018.00775

FIGURE 4.

FIGURE 4

Spatio-temporal localization of HINs during barley endosperm development. (A) Anti-PINa/b labeling of HINs at 12 and ≥20 dap. Note the layers of aleurone (A), subaleurone (SA), and the starchy endosperm (SE) in the bright field channel. At 12 dap, no signal of HINs could be detected in aleurone, whereas a faint signal could be detected in A (arrows) at ≥20 dap. At 12 and ≥20 dap, anti-PINa/b strongly labels protein bodies (PBs) in SA and in SE, respectively (arrowheads). (B) Close-up of a cell representing each tissue. The signal at PBs appears to be constant in SA between 12 and ≥20 dap (arrowheads). Note the signal of HINs at the vacuolar membrane (arrows) in the A at ≥20 dap and the strong signal at the periphery of starch granules in the SE at ≥ 20 dap (open arrowheads). Labeled PBs show reduced sizes in SE at 12 and ≥20 dap (arrowheads). Scale = 100 μm. s indicates starch granules.