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Spatio-temporal HIN transcripts quantification in developing barley endosperm. Bar graph describes the average over three biological replicates of the normalized transcripts from HIN transcripts in aleurone (A), subaleurone (SA), and starchy endosperm (SE) at (A) 12 and (B) ≥20 dap. HIN transcripts in (C) A, (D) SA, and (E) SE at 12 and ≥20 dap. For statistical analyses, we performed a Student’s t-test. Bars represent standard deviation. Note the indicated p-values.
