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. 2018 Jun 8;9:428. doi: 10.3389/fneur.2018.00428

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Copyright © 2018 Riemann, Younsi, Scherer, Zheng, Skutella, Unterberg and Zweckberger.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The distribution of caspase-3-positive cells shows an accumulation of apoptotic cells at the epicenter in injured control animals with apoptotic cell numbers steadily decreasing toward the periphery (n = 6). (B) The quantification of caspase-3-positive cell numbers yields a significant reduction in the NPC group compared to the control group (n = 6–7). (C) Apoptotic cell counts were generally higher in the gray matter and were also significantly reduced in the NPC group compared to the control group. (D–F) Caspase-3-positive cells (red) with additional DAPI-expression (blue) were counted as apoptotic cells, as indicated by the yellow arrows (scale bars = 15 μm).