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. 2018 Jun 3;2018:8521060. doi: 10.1155/2018/8521060

Figure 4.

Figure 4

EEEV-specific antibody responses and survival of vaccinated mice. Female BALB/c mice (n = 10 per group) were vaccinated on days 0, 21, and 42 with 5 μg of empty vector DNA, 5 μg of the EEEV DNA vaccine, or 5 μg each of the VEEV, WEEV, and EEEV DNA vaccines (3-EEV DNA vaccine) delivered by IM EP or 0.5 ml of the formalin-inactivated EEEV IND vaccine delivered by subcutaneous injection. Serum samples obtained on days 21, 42, and 63 were assayed for total IgG anti-EEEV antibodies by ELISA and for EEEV-neutralizing antibodies by PRNT. The group mean log10 ELISA (a) and PRNT80 (b) titers along with the SEM are shown. ∗p < 0.05 for comparison of titers between groups. Four weeks after the final vaccination, the mice were challenged with 1 × 105 PFU (~3000 LD50) of EEEV strain FL91-4679 by the aerosol route. Kaplan-Meier survival curves indicating the percentage of surviving mice at each day of the 28-day postchallenge observation period are shown (c). ∗p < 0.05 for survival rate as compared to negative control group.