Table 3.

Effect of Essential oil of Isodon rugosus on formalin induced pain in mice.

Samples	Dose (mg/kg)	Total time spent in licking
		0–5 min	% Inhibition	15–30 min	% Inhibition
Negative cont.	–	57.33 ± 0.88	–	67.00 ± 0.93	–
Ir.Eo	50	39.50 ± 1.11***	31.10	49.67 ± 1.92***	25.86
Ir.Eo	100	26.16 ± 0.94***	54.36	38.33 ± 0.71***	43.28
Mor	5	11.83 ± 1.24***	79.36	13.67 ± 0.67***	79.59
Mor + Nal	5 + 1	54.67 ± 1.02ns	04.63	63.50 ± 1.17ns	05.22
Ir.Eo + Nal	50 + 1	52.16 ± 0.70**	09.01	61.67 ± 1.14*	07.95

Ir.Eo, Essential oil of Isodon rugosus; Mor, Morphine; Nal, Naloxone; Total time is represented as mean ± SEM.

^*P < 0.05,

^**P < 0.01,

^***P < 0.001.