Table 5.
Mass spectrometry identification of proteins present in spots from saliva 2-DE profiles differing between stimulation conditions and/or among breeds.
| Spots | Protein | Entry reference |
Estim/ theoret MW (kDa) |
Estim/ theor pI |
Score ID∗ | % Seq. Cov. | Matched Peptides MS (MS/MS) |
|---|---|---|---|---|---|---|---|
| 0 | Chain A, Crystal Structure Analysis Of Canine Serum Albumin | pdb|5GHK|A (NCBI) | 78.1/65.7 | 4.9/5.3 | 263 | 42 | 18 (2) |
| 5 | Keratin, type I cytoskeletal 10 | Q6EIZ0 (Uniprot) | 18.5/57.7 | 7.8/5.1 | 207 | 30 | 10 (5) |
| 8 | double-headed protease inhibitor, submandibular gland | XP_022264993.1 (NCBI) | 17.9/15.7 | 6.0/8.6 | 428 | 58 | 4 (8) |
| 12 | Immunoglobulin J chain | XP_532398.2 (NCBI) | 30.1/18.3 | 4.4/4.7 | 125 | 40 | 3 (2) |
| 16 | Immunoglobulin lambda-1 light chain isoform X34 | XP_005636600.1 (NCBI) | 30.0/24.8 | 6.0/6.4 | 326 | 33 | 6 (4) |
| 18 | Immunoglobulin lambda-1 light chain isoform X25 | XP_022266294.1 (NCBI) | 31.0/24.9 | 5.5/5.1 | 198 | 35 | 8 (4) |
| 37 | IgGFc-binding protein | XP_022261796.1 (NCBI) | 59.9/318.0 | 4.9/5.2 | 267 | 4 | 7 (4) |
| 45 | Uncharacterized protein | J9P732 (Uniprot) | 25.0/21.4 | 5.8/6.0 | 192 | 28 | 4 (5) |
| 81 | Uncharacterized protein | F1PW98 (Uniprot) | 19.1/55.0 | 8.0/5.7 | 111 | 29 | 15 (2) |
∗Protein score is −10∗log(P), where P is the probability that the observed match is a random event. Protein scores greater than 62 are significant (p < .05).