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MSC-CM suppresses mineral deposition and ALP activity in VSMCs. VSMCs from different treatment groups were incubated for 7 days. Images of (a) alizarin red staining (magnification ×100) and (b) von Kossa staining (×100). The graphs depict the quantification of alizarin red staining (c), calcium content (d), and ALP activity (e) analyses.
