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. 2018 Jun 3;2018:6053567. doi: 10.1155/2018/6053567

Figure 4.

Figure 4

MSC-CM prevents β-GP-induced VSMC apoptosis. (a) A CCK8 assay was used to test VSMC viability (∗P < 0.05). (b) The frequency of VMSC apoptosis was analysed using flow cytometry and calculated as quartile 2 (Q2) (FITC+/PI+) + Q3 (FITC+/PI−). (c) Apoptotic cells were determined among Hoechst-stained VSMCs (×200). (d) The relative mRNA expression (e) and protein expression of caspase-3 were determined by RT-PCR and western blotting, respectively. (f) The Bcl-2/Bax mRNA expression ratio was determined by RT-PCR. ∗P < 0.05.