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Timeline of 3T3-L1 differentiation and induction of insulin resistance. CS/DMEM: 10% calf serum/Dulbecco's Modified Eagle's Medium; IMI (induction media I): 10% fetal bovine serum (FBS)/DMEM + 0.5 mmol/L isobutylmethylxanthine (IBMX) + 1 μmol/L dexamethasone (Dex) + 10 μg/mL insulin; IMII (induction media II): 10% FBS/DMEM + 1 μg/mL insulin; FBS/DMEM: 10% FBS/DMEM; IMIII (induction media III): 10% FBS/DMEM + 1 μmol/L Dex.
