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. 2018 Jun 20;9:46. doi: 10.1186/s40104-018-0263-z

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — The gene expression levels of CCL8 in peripheral blood leukocytes obtained from cows on 14, 15, 16, 17 and 18 d after artificial insemination (AI). Based on the day of return of estrus, the cows were divided into three groups, pregnant (n = 5), early embryonic mortality (EEM; n = 5) and late embryonic mortality (LEM; n = 5). a Data are means ± SEM of five cows and are expressed as relative ratios of the mRNAs to GAPDH. Values significantly different from the value on 14 d after AI are shown with an asterisk (*P < 0.05). b Each line represents the data for individual cows, and the figure in parentheses indicates the day of the return of estrus in EEM and LEM cows