Fig. 1. Enzymatic reaction of SE1 with esterases. (a) Fluorescence spectra of SE1 (1.0 μM) in PBS buffer (10 mM, pH 7.4, 37 °C) before and after the addition of 0.2 unit porcine liver esterase (PLE), measured every 1 min. (b) Plot of V0versus various concentrations of SE1. (c) Time course of the ratio (Fyellow/Fblue) of SE1 (1.0 μM) after addition of human CE1 and CE2 (approximately 10 units) in PBS buffer.
