Fig. 6. Pseudocolored ratiometric TPM images (Fyellow/Fblue) of rat liver tissues stained with 20 μM SE1 for 2 h (a) before and (c) after pretreatment with 500 μM BNPP for 1 h before labeling with SE1. (b and d) Higher magnification images of (a) and (c). Images were acquired at depths of approximately 300 μm using a 740 nm excitation source and emission windows of 420–470 nm (Fblue) and 520–570 nm (Fyellow). Scale bars = (a and c) 48 and (b and d) 19 μm. (e) The average emission ratio (Fyellow/Fblue) in (a and c).
