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. 2018 Jan 2;5(4):678–690.e1. doi: 10.1016/j.jcmgh.2017.12.012

Figure 2.

Figure 2

IL-17A induces the caspase-dependent death of corpus gastroids in vitro. Corpus glands were isolated and cultured in Matrigel in gastroid-forming conditions. Red arrows denote degenerated organoids. (A) Gastroids were cultured for 48 hours and then media was supplemented with IL-17A. Gastroid death was compared between IL-17A supplemented and controls. (B) Representative immunofluorescent images of control and IL-17A-treated organoids stained with Hoechst (blue) and TUNEL (green). (C) Gastroids were cultured for 48 hours and then media was supplemented with IL-17A or IL-17A + a pan-caspase inhibitor, ZVAD-FMK, or IL-17A + a necroptosis inhibitor, necrostatin-1. Red arrows denote dead organoids in each field of view. (D) Percentage of degenerated organoids in control, IL-17A-treated, IL-17A + ZVAD-FMK, and IL-17A + necrostatin-1 conditions. Data are the means ± SEM of 3 experiments with 8–12 cultures per group. Significance was calculated using the Student t test.