Figure 3.

Parietal cells express IL-17A receptor. (A) Representative immunofluorescent staining of healthy BALB/c (2 months old) mouse gastric corpus for Hoechst (blue), VEGF-B (red), and IL-17RA (yellow). A magnification pane is outlined in white showing the expression of IL-17RA on VEGF-B–expressing parietal cells. (B) Image cytometry software was used to quantify the intensity of IL-17RA fluorescence on parietal cells using immunofluorescent images. Shown is a representative plot of VEGF-B–positive parietal cells (red) and VEGF-B–negative cells (blue) with histograms of IL-17RA staining intensity in each of these groups. Epithelial cells isolated from the gastric corpus of BALB/c mice (2 months old) were cultured in media or media supplemented with IL-17A for 24 hours. Cytospin slides were generated from control and treated glands for microscopic analysis. (C) Representative immunofluorescent staining of Cytospins generated from cultured gastric epithelial cells stained with Hoechst (blue), anti–VEGF-B (red), and TUNEL (green). (D) TUNEL⁺ parietal cells were quantified as a percentage of total parietal cells (PCs). Data are means ± SEM of 10 representative images prepared from 6 slides over 2 separate experiments.