Fig. 2.

Fulvestrant-resistant cells exhibit a more motile mesenchymal phenotype compared to parental fulvestrant-sensitive cells. The motility of MCF-7-based fulvestrant-sensitive and fulvestrant-resistant cells was evaluated by a transwell assay. a Representative micrographs (purple-stained cells, × 20 magnification) and b column diagram analysis of the percentage of cells that migrated through the membrane: *p < 0.05. Data are shown with error bars representing mean ± standard deviation. c Protein expression levels of E-cadherin, vimentin and estrogen receptor (ER)α by western blotting. β-actin was used as loading control. A representative of three independent experiments is shown