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. 2018 Jun 19;16:53. doi: 10.1186/s12951-018-0379-5

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — MUC5AC immunofluorescence staining of the HT29-MTX cell line. Representative confocal laser scanning microscopy z-stack image of MUC5AC (in red) produced by HT29-MTX cells (E-cadherin, in green) cultured under standard conditions. A z-stack section (x, y) at the level of the mucus; B z-stack section (x, y) at the level of HT29-MTX cells with orthogonal x (y, z) and y (x, z) sections on the right and bottom, respectively. The image was prepared using the easy 3D section view of IMARIS software with extended view (signal gathered from a thickness of 5 µm)