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. 2018 May 17;14:1744806918781259. doi: 10.1177/1744806918781259

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© The Author(s) 2018

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 4. — The contact of VGLUT1-positive terminals with neurons of the NAc was decreased. (A) Double immunostaining for VGLUT1 (red, (a) and (b)) and MAP2 (green, (c) and (d)) in the NAc. (e) and (f) are merged figures of (a) and (c), and of (b) and (d), respectively. Insets are enlarged image of dashed areas in each figure. Arrowheads indicate VGLUT1-positive terminals contacting with MAP2-positive structure. (B) Double immunostaining for VGLUT2 (red, (a) and (b)) and MAP2 (green, (c) and (d)) in the NAc. (e) and (f) are merged figures of (a) and (c), and of (b) and (d), respectively. Insets are enlarged image of dashed areas in each figure. Arrowheads indicate VGLUT2-positive terminals contacting with MAP2-positive structure. (C and D) Quantitative analysis revealed that the immunostaining density of VGLUT1-positive terminals and the contact between VGLUT1-positive terminals and MAP2-positive neuronal processes were decreased in the CFA group compared with saline group. (E and F) Quantitative analysis revealed that the immunostaining density of VGLUT2-positive terminals and the contact between VGLUT2 and MAP2-positive structures were unaltered in the CFA group compared with saline group. Scale bar = 10 µm. The data are presented as the means ± SEM (n = 5/group *p < 0.05, compared to the saline group).

VGLUT: vesicular glutamate transporters; NAc: nucleus accumbens; MAP2: microtubule-associated protein 2; CFA: complete Freund’s adjuvant; SEM: standard error of mean.