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Interaction of transcription factors with the E-box element of the IKBL-62*1 promoter and the IKBL-421 promoter sequences in Jurkat cells. (A) 32P-Labeled probes (25 fmol) with Jurkat nuclear extract (3 μg). Molar excess (25-, 50-, and 100-fold) of unlabeled oligonucleotide added to reactions containing probe IKBL-62*1 established specificity. No protein binding was detected on allele 2. (B) Polymorphism at position −421 did not alter the binding of nuclear proteins.
