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Consensus sequences suggest δEF1, USF1, and E47 bind to IKBL-62*1 probe. 32P-labeled IKBL-62*1 probe (25 fmol) with Jurkat nuclear extract (3 μg). Excess (100-fold) unlabeled consensus oligonucleotides for the transcription factors δEF1-A, USF1-B, and E47-C inhibit bands specific to IKBL-62*1 probe. Consensus oligonucleotides for NFκB and AP2 lack an E-box binding motif and were not inhibitory.
