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Kinetics of IKBL and TNFA mRNA expression in Jurkat cells after PMA/Ca2+ ionophore induction. Jurkat cells were treated with PMA/Ca2+ ionophore (20 ng/μl); samples were harvested at 0, 1, 3, 6, and 24 h and assayed for endogenous IKBL and TNFA mRNA levels by RT-PCR. Effective mitogenic stimulation was confirmed by a peak in TNFA mRNA expression at 3 h, coincident with a 60% decrease in IKBL expression.
