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. 2018 Jun 20;12(2):69–81. doi: 10.3727/000000005783992124

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Comparison of Pyrosequencing and real-time PCR method for quantifying Gαs splice variants. Three retinoblastoma and three CLL cDNAs were analyzed for Gαs total to Gαs long ratios. The same cDNAs were used for comparing Pyrosequencing and real-time PCR results. Pyrosequencing results in 5.36 ± 0.093 ratio for CLL and 2.49 ± 0.158 for retinoblastoma (p < 0.0001), whereas real-time PCR using SYBR green leads to 4.79 ± 0.382 and 2.51 ± 0.326 (p < 0.05), indicating Pyrosequencing to be the more precise method for quantification of splice variants.