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. 2018 Jun 20;12(3):193–212. doi: 10.3727/000000005783992052

Figure 3.

Figure 3

The CCAAT box functions as a negative cis-regulatory element when different cis-regulatory elements of the FGF-4 enhancer are disrupted. Transfection of F9 EC cells with 15 μg of the indicated plasmid. Results are presented as fold induction over the activity of the constructs that contain a wild-type CCAAT box. Each construct that contains a wild-type CCAAT box was set to 1. This experiment was performed five times. The data shown are the compilation of the five experiments, and the standard deviation for each condition is represented by error bars. Analysis of the data by the Student’s t-test indicated that the activity of CCAATmut+EGTmut was significantly greater than the activity of 427T+EGTmut with a p-value of less than 0.05, the activity of CCAATmut+EDSHMGmut was significantly greater than the activity of 427T+EDSHMGmut with a p-value of less than 0.05, and the activity of CCAATmut+EnGSp was significantly greater than the activity of 427T+EnGSp with a p-value of less than 0.05.