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. 2018 Jun 20;12(3):193–212. doi: 10.3727/000000005783992052

Figure 4.

Figure 4

The CCAAT box functions as a positive cis-regulatory element when p300 is tethered to the FGF-4 enhancer or in the presence of the SV40 enhancer. (A) Transfection of F9 EC cells with 15 μg of 427T+EnGSp or CCAATmut+EnGSp alone or with 5 μg of Gal4/p300. Results are presented as fold induction over the activity of 427T+EnGSp, which was set to 1. This experiment was performed three times. The data shown are the compilation of the three experiments, and the standard deviation for each condition is represented by error bars. Analysis of the data by the Student’s t-test indicated that the activation of CCAATmut+EnGSp by Gal4/p300 was significantly less than the activation of 427T+EnGSp by Gal4/p300 with a p-value of less than 0.05. (B) Transfection of F9 EC and F9-differentiated cells with 15 μg of 427T+SV40E or CCAATmut+SV40E. Results are presented as CAT expression relative to the activity of 427T+SV40E, which was set to 1. This experiment was performed four times. The data shown are the compilation of the four experiments, and the standard deviation for each condition is represented by error bars. Analysis of the data by the Student’s t-test indicated that the activity of CCAATmut+SV40E was significantly less than the activity of 427T+SV40E in both F9 EC and F9-differentiated cells with a p-value of less than 0.05.