Skip to main content
. 2018 Jun 20;12(3):193–212. doi: 10.3727/000000005783992052

Figure 7.

Figure 7

The orientation and the position of the CCAAT box is important for the regulation of the FGF-4 promoter. (A) Physical map of the promoter/reporter gene constructs that contain a reversed or upstream CCAAT box. (B) Transfection of F9 EC cells with 15 μg of 427T+E, CCAATmut+E, invert CCAAT+E, and upstream CCAAT+E. Results are presented as CAT expression relative to the activity of 427T+E, which was set to 1. This experiment was performed five times. The data shown are the compilation of the five experiments, and the standard deviation for each condition is represented by error bars. Analysis of the data by the Student’s t-test indicated that the activity of CCAATmut+E and upstream CCAATmut+E was significantly less than the activity of 427T+E with a p-value of less than 0.05, whereas the activity of reversed CCAAT+E was not significantly different than the activity of 427T+E. (C) Transfection of F9 EC cells with 15 μg of 427T, CCAATmut, invert CCAAT and upstream CCAAT. Results are presented as fold induction over the activity of 427T, which was set to 1. This experiment was performed four times. The data shown are the compilation of the four experiments, and the standard deviation for each condition is represented by error bars. Analysis of the data by the Student’s t-test indicated that the activity of CCAATmut, reversed CCAATmut, and upstream CCAATmut were significantly greater than the activity of 427T with a p-value of less than 0.05.