TABLE 3.

HEPATIC GENE EXPRESSION MODULATIONS INDUCED BY FENOFIBRATE OR LGD1069 IN WILD-TYPE AND PPARα −/− MICE

Genes	Wild-Type		PPARα −/−		CT PPARα −/−/wt	Function
	Feno/CT	LGD1069/CT	Feno/CT	LGD1069/CT
Cyp4al0	32.9†	7.8†	ns	ns	ns	microsomal FA ω-hydroxylation
Cyp4al4	44.8†	12.0†	ns	ns	2.4†	microsomal FA ω-hydroxylation
AOX	6.4†	2.5†	ns	ns	ns	peroxisomal FA oxidation and biogenesis
BIEN	40.0†	5.1†	ns	ns	ns	peroxisomal FA oxidation and biogenesis
THIOL	8.5†	3.8†	ns	ns	ns	peroxisomal FA oxidation and biogenesis
PECI	2.1†	ns	ns	ns	ns	peroxisomal FA oxidation and biogenesis
PMDCI	3.2†	ns	ns	ns	ns	peroxisomal FA oxidation and biogenesis
Pex11a	2.1†	ns	ns	ns	ns	peroxisomal FA oxidation and biogenesis
CACP	1.8†	ns	ns	ns	ns	mitochondrial FA oxidation and energy homeostasis
CPT2	1.7†	ns	ns	ns	ns	mitochondrial FA oxidation and energy homeostasis
MCAD	2.8†	ns	ns	Ns	1.6*	mitochondrial FA oxidation and energy homeostasis
Tpα	2.4†	ns	ns	ns	ns	mitochondrial FA oxidation and energy homeostasis
mHMGCoAS	3.1†	1.5*	ns	ns	ns	mitochondrial FA oxidation and energy homeostasis
PDK4	3.3†	ns	ns	ns	ns	mitochondrial FA oxidation and energy homeostasis
ACOTH	6.3†	2.0*	ns	ns	ns	FA metabolism
FAT/CD36	1.91†	ns	ns	ns	ns	FA transport
L-FABP	4.2†	ns	ns	ns	ns	FA trafficking
FAS	ns	3.5†	ns	2.4†	ns	lipogenesis
S14	ns	2.4†	ns	ns	Ns	lipogenesis
Cyp26	ns	3.5†	ns	4.4†	ns	other cytochromes P450
Cyp2bl0	ns	4.6†	1.7†	3.3†	ns	other cytochromes P450
Cyp2c29	−1.6†	2.2†	ns	1.7†	1.5†	other cytochromes P450
Cyp8bl	1.8*	−2.1†	ns	ns	ns	other cytochromes P450
ALDH3	4.1†	ns	ns	ns	ns	fatty aldehyde dehydrogenase
GSTμ	ns	2.2†	ns	ns	ns	glutathione S-transferases
GSTπ2	ns	−3.6†	ns	ns	−2.9†	glutathione S-transferases
LCE	−1.6†	ns	ns	ns	ns	TG hydrolysis
PLTP	2.0†	ns	ns	ns	ns	lipoprotein metabolism
apoC-III	ns	ns	ns	ns	1.6†	lipoprotein metabolism
PPARα	1.6†	ns	ns	ns	ns	nuclear receptor
SPI1	−2.7†	−2.1†	ns	−1.6*	−1.6*	acute phase response
ADISP/FSP27	2.0†	ns	ns	ns	ns	apoptosis
BSEP	ns	ns	ns	1.9†	ns	bile salt transport

Hepatic total RNA from wild-type and PPARα −/− mice treated with fenofibrate (Feno), LGD1069, or vehicle alone (CT) were hybridized to INRArray 01.2 (n = 3 or 4 per group, total of 22 macroarrays). Raw data were normalized by the mean log(signal) of eight artificial mRNAs spiked into the samples. Normalized data were subjected to an ANOVA followed by a two-tailed Student’s test using a Bonferroni comparison-wise error rate protection. The ratios (fold change) of transcript abundance correspond to the comparisons between the groups indicated in the headings of the columns. Genes presented exhibited at least one highly significant (p < 0.002) gene expression modulation of 1.5-fold amplitude minimum out of the five comparisons studied. wt: wild-type.

Due to the Bonferroni p-value adjustments: *raw p <0.01 and †raw p < 0.002. ns: a nonsignificant expression ratio (raw p > 0.01).