TABLE 4.
VERIFICATION OF GENE ARRAY RESULTS BY REAL-TIME QUANTITATIVE PCR ANALYSIS
| Gene (Accession No.) | Relative Gene Expression | |
|---|---|---|
| cDNA Array | Real-Time PCR | |
| Peroxisome proliferative activated receptor γ (N89673) | −4.0 | −6.6 |
| Dickkopf homolog 3 (AA425947) | −5.7 | −5.4 |
| LIM protein (R92455) | 3.4 | 12.2 |
| Insulin-like growth factor-2 (N74623) | 8.8 | 91.7 |
| Human insulin-like growth factor binding protein 5 (T52830) | 4.5 | 10.3 |
Gene expression measured with both techniques shows good correlation. Differences in expression levels seem to be more pronounced with real-time PCR analysis.